Core PlatformStabilized Reagent ChemistryTime-Gated Single-Read Detection

A stabilized-reagent platform for practical online free chlorine monitoring

OneStep Technologies is developing a stabilized chromogenic reagent platform for automated water analysis, with a current focus on online free chlorine monitoring. The platform combines stabilized single-reagent chemistry with time-gated single-read optical detection to support practical, low-maintenance operation.

Reaction profiles are evaluated during method development to define a predetermined measurement time. Routine quantitation subsequently requires one absorbance reading per analytical cycle, without slope fitting, derivative calculations, or regression-window selection.

Together with reagent stability of up to 24 months under sealed storage conditions, this architecture is designed to simplify operation, reduce routine maintenance workload, and support integration into instrument-based monitoring systems used in real-world water-analysis environments.

Stabilized reagent chemistry
The platform is built around stabilized TMB-derived chromogenic chemistry designed for extended usability, repeatable performance, and practical deployment in automated analytical systems.
Time-gated single-read detection
A predefined measurement gate enables routine quantitation from one absorbance reading per analytical cycle, eliminating the need for routine slope fitting, derivative analysis, or multi-point regression.
Simplified reagent workflow
The single-reagent architecture is designed to reduce operational complexity and support lower-maintenance use in automated online monitoring applications.
Instrument integration
The platform is designed for integration into flow-cell optical analyzers and other reagent-based water-monitoring instruments.
What problem it addresses
Conventional online chlorine workflows can involve multiple reagents, limited reagent stability, frequent intervention, and higher maintenance requirements. The platform is designed to support a more practical operating model.
Why it is different
The technical differentiation lies in combining stabilized single-reagent chemistry with a predefined-time single-read measurement architecture designed specifically for automated online monitoring.
Why it matters
For instrument companies and commercial partners, analytical chemistry must also be stable in operation, straightforward to manufacture, easy to deploy, and compatible with real monitoring systems.

From reaction-profile development to routine single-read operation

Time-resolved reaction profiles are used during development to identify a measurement window that provides a reproducible free chlorine response while limiting contributions from more slowly reacting combined chlorine species.

Once the measurement time is established, the routine analyzer workflow is simplified to a single optical reading at the predefined time. This separates development-stage reaction characterization from routine measurement and signal processing.

Development stage
Time-resolved reaction data are evaluated to define the measurement gate and characterize timing, temperature, concentration, and interference effects.
Routine measurement
The analyzer performs one absorbance measurement at the predefined time during each analytical cycle.
Deployment objective
The combined reagent and measurement architecture is intended to support reliable online monitoring with fewer routine interventions.